Sterilization methods pdf

The sterilization methods discussed include steam sterilization , ethylene oxide (ETO), hydrogen peroxide gas plasma, and liquid peracetic acid. Classical sterilization techniques using saturated steam under pressure or hot air are the most reliable and should be used whenever possible. Other sterilization methods include filtration , ionizing radiation ( gamma and electron-beam radiation ), and gas ( ethylene oxide , formaldehyde). Sterilization is a complex process.

It is imperative that healthcare workers and technicians involved in the reprocessing and sterilization of medical devices have a thorough knowledge and understanding of the scientific principles and methods of sterilization utilized in today’s health care settings. The only methods for many heat sensitive products especially biologics, antibiotics, oncology products and othersoncology products and others.

Requires a subsequent aseptic process Removes but does not destroy microorganisms so it must be considered separately from the other methods. Method Mechanism Conditions Uses 1. Dry heat sterilization (hot air oven) Oxidation Requires 170˚c temperature for hr. Oily materials, powders, glass syringes , needles 2. A) Moist heat sterilization Autoclave Protein denaturation At 15psi of pressure(121˚c) for min.

Heat sterilization is the most widely used and reliable method of sterilization , involving destruction of enzymes and other essential cell constituents. The process is more effective in hydrated state where under conditions of high humidity, hydrolysis and denaturation occur, thus lower heat input is required. This method requires post-vacuum and dry cycles.

The instruments should rest on a sterilizer rack for minutes to prevent water condensation on the lens Gas sterilization Using ethylene oxide (EO) is suitable for all disposable instruments, insulated hand instruments and tubings used for gas,. What is the best sterilization technique to use? Is disinfection more effective than sterilization?

What chemicals are used in sterilization? What are methods of cold sterlization? Thermal (Heat) methods B. Ethylene oxide sterilization is an important sterilization method that manufacturers widely use to keep medical devices safe. One method of sterilization is to injecting n into the fermenter mantle or interior coils red sterilization. Another method is to inject steam into the nutrient solution itself.

With direct steam injection, condensate accumulates in the fermenter and the volume of liquid increases during the sterilization process. To ensure the autoclaving process was able to cause sterilization , most autoclaves have meters and charts that record or display pertinent information such as temperature and pressure as a function of time. Conventional method: Packaging is done before heat treatment. It is also known as In-bottle sterilization.

UHT or aseptic method: Packaging is done after heat treatment. NeWer methods oF sterilization A priority for hospitals with high workload is the rapid turnaround times for instruments that cannot be sterilized satisfactorily with steam or dry heat. STERILIZATION TECHNIQUES 2. The heat used kills the microbes in the substance.

This is the most common method of sterilization. The preferred method of control is elimination of the chemical (through engineering controls or substitution) or relocation of the worker. The following overview of the performance characteristics of each provides users with sufficient information to select an appropriate disinfectant for any item and use it in the most efficient way.